Production of hybrid seed using GA3 and genetic markers in tomato (Lycopersicon esculentum Mill.)
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Date
2005
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CCSHAU
Abstract
The present study was conducted at CCS HAU, Hisar
for production of hybrid seeds using different concentration of
GA3 and genetic markers. Five lines, i.e., selection-21 (potato leaf
type), CLN-2366A, CLN-2116D9F1-180-31-9-40,
CLN-2-16-8-2-176 and EC-339357 (anthocyaninless type) were
used as female parent. Three lines Hisar Arun, H-86 and VFN-8
were used as male parent are cut leaf type and anthocyanin
present on the hypocotyl. Cut leaf type is dominant over potato
leaf type and anthocyanin type is dominant over anthocyaninless
type. GA3 was sprayed 40 and 60 days after transplanting as
whole plant spray @ 50 and 100 ppm. Fifteen different crosses
were made and observations for per cent fruit set, number of
seeds per fruit, seed germination, seed vigour index I and II and
percent hybrids plants. Confirmation of markers was done at
early stage of seedling in the green house.
Among all the treatments, the GA3 50 ppm was found
best for fruit setting and seed germination. However, for the
number of seeds per fruit and seed vigour index I and II, hand
emasculation and hand pollination was at par with GA3 50 ppm.
The per cent hybrid plants identified at seedling stage were non
significant under different treatments. However, cost of producing
hybrid seed was 38% less in GA3 50 ppm as compared to hand
emasculation and hand pollination.
The futuristic approach of hybrid seed production
using different concentrations of GA3 on different genetic markers
and ancillary investigations have been suggested.