Assessment of diversity between male and female plants of date palm (Phoenix dactylifera L.) using ISSR markers
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Date
2006
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CCSHAU
Abstract
Date Palm is an important crop. It is considered as a fresh fruit. It ranks five in
the production list of tropical and sub-tropical fruits after citrus, mangoes,
bananas and pineapples. As a dried fruit, dates easily top the list over raisins, figs
and prunes. It is a dioecious plant. This study was conducted to prepare DNA
fingerprint database of 30 genotypes of Date Palm to assess the diversity between
male and female plants so that sex of the plant could be determined at the
juvenile stage. Out of total 44 ISSR primers used in this investigation, 34 primers
amplified while 24 primers showed polymorphism. Using these 24 primers 193
alleles were obtained, out of which 139 bands were polymorphic and 54 bands
were monomorphic.
Analysis of this polymorphism profile generated using NTSYS-PC
package, the 30 date palm genotypes were grouped separately into two clusters.
Group I consisted of all male plants and females of Khadrawi. Group II consisted
of all females of Shamran variety.
The similarity matrix further showed the relatedness among 30 genotypes.
A high polymorphism value of 71.08+1.49 among all the genotypes was detected.
Also a moderately high polymorphism was observed among the males
(71.24+2.98%), Shamran variety females (70.76+2.94%) and Khadrawi variety
females (75.61+3.04%).
The polymorphic study of Date Palm has revealed that one primer (KJ-12)
can be a putative sex linked marker and needs further verification by using more
male plants and female plants of Date Palm.