Studies on myb gene expression in Indian mustard (Brassica juncea L. Coss. & Czern) under salt stress
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Date
2007
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Publisher
CCSHAU
Abstract
The investigation was carried out to study the myb expression under different
levels of salinity in Brassica juncea cv. CS52 in the Department of Biotechnology &
Molecular Biology, CCS Haryana Agricultural, Hisar during the year 2006 and 2007.
The myb gene expression was checked by RT-PCR approach. Primers BjMYBR-1 and
BjMYBR-2 were designed from the conserved DNA binding domain of Atmyb2 by
analyzing this gene sequence with other myb gene sequence available in database
using ClustalW programme. The expression of Bjmyb1 became detectable within 15
minutes of salinity exposure, which increased and was maximum in 30 minutes of
salinity treatment. Thereafter it declined and stabilized in 2 hr of salinity treatment.
The expression was not affected by the different concentrations (100 mM, 200 mM,
300 mM and 400 mM) of NaCl used. Sequencing of amplified cDNA fragment revealed
a 176 bp sequence. ClustalW analysis of Bjmyb1 with other eight myb genes from
different organisms available in database showed its homology with Atmyb2 and
Chmyb2.
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Keywords
Masturd, Brassica juncea L. (Coss. and Czern), RT-PCR, salinity stress, Myb gene expression, Transcription factor, Gel electrophoresis.