Genetic and symbiotic characterization of poly-β-hydroxybutyrate production in Rhizobium and Bradyrhizobium spp. nodulating mungbean [Vigna radiata (L.) Wilczek]
Loading...
Date
2008
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Twenty-one WT strains of Rhizobium/Bradyrhizobium sp. (Vigna) were isolated
purified and tested for their ability to form nodules on the mungbean plants. These WT
strains were screened for PHB production on Beringer’s MM with Nile blue A under
UV light. The two WT strains MBR 16 and MBR 25 produced high amount of PHB
and these were used to isolate PHB mutants. Three types of mutants were isolated and
characterized as MHt, MM and ML mutants. The MHt mutants produced highest
amount of PHB, whereas ML mutants produced very low amount of PHB. The
Maximum amount of PHB was found in MBR 16 MHt 1 i. e. 1.24 g/l.
The antibiotic resistance pattern of parent strains and their mutants was almost
same. The symbiotic effectivity of parent strains and their mutants on mugbean plants
showed that the MHt mutants had higher shoot dry weight and total shoot nitrogen than
the MM and ML mutants. A highly significant positive correlation was found between
the amount of PHB produced and shoot dry weight and total shoot nitrogen of the
munbean plants.
Description
Keywords
Rhizobium/Bradyrhizobium, PHB production, antibiotic resistance symbiotic efficiency