In vitro regeneration studies in garlic (Allium sativum L.) cvs. HG-17 and G-282
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Date
2009
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Publisher
CCSHAU
Abstract
Garlic is used worldwide as a spice and a condiment. It has
very high nutritive value and is a valuable foreign exchange earner for
India. It is well known throughout history for its medicinal properties. It
is sexually sterile, hence it is propagated vegetatively. During present
investigation, in vitro regeneration in garlic cv. G-282 and HG-17 was
studied. Basal part of a vertically cut clove was used as an explant. 2iP
was used at 1, 1.5, 3 and 3.5 mg/l while TDZ was used along with 2iP at
0.1, 0.2 and 0.3 mg/l keeping concentration of 2iP constant at 0.1 mg/l.
Highest percentage of multiple shoot formation in both G-282 (90.3%)
and HG-17 (80.5%) was observed with 3 mg/l 2iP. The average number
of shoots produced was highest with MS + 3 mg/l 2iP for both G-282 (8.9
shoots/ explant) and HG-17 (7.5 shoots/explant). IAA (1.5 mg/l) induced
maximum percentage of roots for both G-282 (75.0%) and HG-17
(77.4%). Average number of roots was highest in MS + 1.5 mg/l IAA for
G-282 (9.9 roots/shoot) and HG-17 (9.5 roots/shoot). 0.5 mg/l NAA + 0.5
mg/l IBA was found to be the ideal concentration for induction of callus
using young leaf explants. MS basal medium containing 10% sucrose
induced maximum bulblet formation for both G-282 (87.5%) and HG-17
(75.0%). 41.7% of the regenerated plants of the cultivar G-282 and 50.0%
of the regenerated plants of the cultivar HG-17 survived under pot
conditions.
Description
Keywords
Garlic, Regeneration, Vegetative propagation, Planting, Biological phenomena, Cloves, Auxins, Allium sativum, Biological development, Cytokinins