Study on Association of Growth Hormone Gene Polymorphism with Milk Production in Cattle
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Date
27-12-12
Journal Title
Journal ISSN
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Publisher
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
Abstract
The genetic variation at the growth hormone gene in a HF cross bred population
and to study the possible association of the identified polymorphism with first lactation
milk yield. Two fragments of bovine growth hormone gene covering part of intron 3 and
exon 5 were PCR amplified from 50 HF cross bred cattle and subjected to RFLP with
MspI and AluI enzymes respectively.
PCR-RFLP of exon 5 of growth hormone gene with AluI yielded monomorphic
pattern of 223, 171 and 52 bp fragments. The sequencing of PCR product confirmed the
pattern as heterozygous (LV). No homozygotes were observed in the population. The
PCR-RFLP of intron 3 region of growth hormone gene with MspI yielded three
genotypes. The frequency of genotypes Msp (+/+), Msp (+/-) and Msp (-/-) were 0.28,
0.12 and 0.60 respectively. The population was not in Hardy Weinberg Equilibrium with
respect to Msp polymorphism. The average first lactation milk yield of HF cross bred
cows with genotypes Msp (+/+), Msp (+/-) and Msp (-/-) were 2493 kgs, 2473 kgs and
2558 kgs respectively and were not significantly different indicating that the SNP had no
influence on the first lactation milk yield in the population studied.
Description
PG. Thesis
Keywords
Study on Association of Growth Hormone Gene Polymorphism with Milk Production in Cattle