DEVELOPMENT OF DROUGHT RESPONSIVE EXPRESSED SEQUENCE TAG (EST) LIBRARY IN Prosopis cineraria (L.) DRUCE
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Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
IARI, NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY
Abstract
To understand the adaptability of Prosopis cineraria (L.) Druce to drought, genes
expressed under drought stress needed to be identified. A step towards characterization of the
genes that contribute to combating abiotic stress in P. cineraria, drought responsive cDNA
library was constructed and analyzed. One-month-old seedlings were subjected to drought
stress by growing them in PEG 6000 (35%) for a period of 10 days. The Relative Water
Content (RWC) of the seedlings was studied to estimate the drought stress and it was
observed to be in the range 79.38 % to 58.33% over a period of 10 days of drought stress.
The resulting library consisted of 564 good quality reads ESTs of which 164 showed
significant homology to genes expressed in stress response. The drought stress responsive P.
cineraria library had 46 unigenes, 28 genes involved in diverse biological functions, 15 were
genes for hypothetical function and 3 genes of unknown function.
Description
T-8429
Keywords
drought stress, Prosopis cineraria, EST, RWC, PEG 6000.