Identification of hybrids and their parents through SSR profiling in maize [zea mays (L.)]
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Date
2016
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Publisher
CCSHAU
Abstract
Zea mays (L.), commonly known as maize or corn, is a diploid grain crop with
20 chromosomes (n=10) belonging to the family Poaceae. It is an important staple food in
many countries and is also used in animal feed and has many industrial applications. The
introduction of new hybrid seeds that can survive low winter conditions, off-season diseases
and pests with high productivity has made maize a profitable alternative for small farmers.
Higher genetic purity is an essential pre–requisite for commercialization of any hybrid because
mixing of hybrids can decrease the production rate. So, clear cut identification is essential for
protection and prevention of unauthorized commercial usage. The SSR markers are preferred
molecular markers for genetic purity identification in many crops due to their high efficiency,
co-dominance, reproducibility, simplicity and accessibility to laboratories. Total seven maize
hybrids along with their ten parents were used in the study for identification of maize hybrids
and their parents. Genomic DNA of maize hybrids (HM 5, HM 8, HM 9, HM 10, HM 11, HM
12, HM 13) and their respective parents (Hki 1344, Hki 1348-6-2, Hki 1105, Hki 161, Hki
1128, Hki 193-2, Hki 163, Hki 1378, Hki 488 IPG, Hki 193-1) were isolated using CTAB
method. A total of 45 SSR primer pairs were used for amplification of the isolated genomic
DNA. Gel electrophoresis was carried out for the separation and detection of the amplified
products. Out of 45 SSR primer pairs used, 3 SSR primers could distinguish the different
hybrids and their parents. The study will be very useful in detecting unwanted seed mixed with
hybrid seed.
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Keywords
Hybrids, Maize, Genetics, Dna, Purity, Polymorphism, Sowing, Genetic structures, Planting, Alleles