PROTECTIVE EFFECT OF CATECHIN ON BIOCHEMICAL AND OXIDATIVE STRESS PARAMETERS FOLLOWING REPEATED ADMINISTRATION OF CHLOROPYRIFOS AND ALUMINIUM IN RATS.
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Date
2016
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Division of Veterinary Pharmacology and Toxicology, F.V.Sc & A.H, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, R.S. Pura.
Abstract
The present study was designed to evaluate the effects of chlorpyrifos, aluminium and catechin alone, and in combinations on various biochemical and oxidative stress parameters in wistar rats. Rats of 150-200g body weight were divided into nine groups of six animals each and were subjected to various daily oral treatment regimes for 28 days. Group I and group II served as controls and received corn oil and CMC respectively, group III recieved chlorpyrifos @ 27mg/kg b.wt, whereas animals in group IV were administered with aluminium chloride @ 38.5 mg/kg b.wt. Group V received combination of chlorpyrifos @27 mg/kg b.wt and aluminium chloride @38.5 mg/kg b.wt, group VI received catechin @20mg/kg b.wt in CMC, group VII received chlorpyrifos @ 27 mg/kg b.wt and catechin @ 20 mg/kg b.wt and group VIII were treated with aluminium chloride @ 38.5 mg/kg b.wt along with catechin@20 mg/kg b.wt. Group IX received chlorpyrifos @ 27 mg/kg b.wt along with aluminium chloride @38.5 mg/kg b.wt and catechin @20 mg/kg b.wt. Chlorpyrifos and aluminium alone or in combination caused significant decrease in Hb.values, with elevation in levels of plasma aminotransferases, phosphatases, creatinine and blood urea, and decreased the levels of cholinesterases and plasma protein. Among the oxidative stress parameters, a significant increase in lipid peroxidation and a decrease in activities of GSH, GST, GPx and SOD was observed by administration of both these toxicants either alone or in combination. Ameliorative effect of catechin with co administration of chlorpyrifos was observed with increase in Hb. level and in plasma enzyme ACP, with increase in antioxidant enzymes like GPx in erythrocytes and in GST of liver towards control. While other biochemical and oxidative stress parameters did not showed any modulatory effect.The results of this study indicate that ameliorative effect of catechin was more prominent when given with aluminium chloride in comparison to chlorpyrifos alone and in combination.
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M.V.Sc. Dissertation