Effect of waterlogging, salinity and their interaction on growth, oxidative and carbohydrate metabolism in pigeonpea (Cajanus cajan L. Millsp.) genotypes
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Date
2016
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Publisher
CCSHAU
Abstract
The present investigation entitled “Effect of waterlogging, salinity and their interaction on growth,
oxidative and carbohydrate metabolism in pigeon pea (Cajanus cajan L. Millsp.) genotypes” was aimed to
study the independent and interactive effect of water logging and salinity on growth, oxidative and carbohydrate
metabolism in pigeon pea genotypes. In the first experiment, four genotypes ICPH 2431, PARAS, UPAS 120, HO9
33 were raised under laboratory conditions. The plants were given waterlogging, salinity (30mM NaCl) and
waterlogging + salinity (30mM NaCl) treatments for 8 and 12 days. After water logging and salinity treatments the
seeds were placed on moist filter paper in petri plates for eight days and observations like germination percent,
seedling fresh weight, dry weight and vigor index were recorded at the end.
Second experiment was conducted with the four genotypes ICPH 2431, PARAS, UPAS 120, HO9 33 in
the screen house to study the effects of waterlogging and salinity and combination on various physiological and
biochemical characteristics. The plants were raised in polythene bags filled with half kg soil + FYM manure
mixture (3 soil: 1manure w/w), NPK (@20:40:20 kg per ha). Twenty and forty days after sowing the polythene
bags were placed in cemented tanks (length 160 cm, breadth 125 cm and depth 65 cm) and treatments were given
to the plants [i.e. waterlogging, salinity (30mM NaCl), waterlogging + salinity (30mM NaCl)] for 8 and 12 days.
After recovery periods of 1 and 8 days various observations like percent survival, chlorophyll content, chlorophyll
fluorescene, electrolyte leakage, lipid peroxidation, RWC, proline, superoxide radical content, H
2O2
content,
antioxidative enzyme activity, antioxidant content, total sugar content, reducing sugar content, non reducing sugar
content, activity of enzymes of carbohydrate metabolism, aerenchyma formation in roots, total plant biomass, yield
and its attributes were measured. Waterlogging, salinity and waterlogging and salinity in combination resulted in
decline of germination percent, seedling fresh weight, dry weight, vigor index, percent survival, chlorophyll
content, chlorophyll fluorescence, RWC, total sugar content, non reducing sugar content, total plant biomass, seed
test weight and seed yield per plant and increase in electrolyte leakage, lipid peroxidation, proline content,
superoxide radical content, H2O2 content, antioxidative enzymes, antioxidant content, reducing sugar contents,
carbohydrate metabolism enzymes, aerenchyma formation in roots. Waterlogging and salinity in co mbination
showed maximum adverse effects however salinity alone showed minimum adverse effects. Salinity had no effect
on percent survival and aerenchyma formation. Forty day old plants were found more sensitive as compared to 20
day old plants. Roots were found more prone to waterlogging, salinity and combined stress. ICPH 2431 followed
by PARAS was found more tolerant among the genotypes under all treatments in terms of indices of stress,
oxidative metabolism, carbohydrate metabolism and yield parameters.
Description
Keywords
Planting, Soil water regimes, Genotypes, Enzymes, Vegetative propagation, Tolerance, Pigeonpeas, Biological phenomena, Sugar, Food preservation