ISOLATION AND SCREENING FOR NOVEL BIOACTIVE MOLECULES FROM ENDOPHYTIC POPULATION ASSOCIATED WITH ARISAEMA ERUBESCENS
Loading...
Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Division of Microbiology, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu
Abstract
Arisaema erubescens is one of the medicinal herbs found abundantly in the
Himalayan region. Healthy plant samples of Arisaema erubescens were collected from
Patnitop situated at an elevation of 2,065 metres (6,640 ft), with 33°05’15.60”N and
75°19’49.91”E coordinates of District Udhampur in Jammu Division. Total nine bacterial
and eight fungal endophytes were isolated from plant samples. Bacterial endophytes were
identified on the basis of Morphological & Biochemical behaviour. Later on promising
cultures were subjected to 16SrRNA tools for species identification. Fungal endophytes
were identified on the basis of morphological & microscopic characteristics and most of
them were of Aspergillus genus. Endophytes were screened for their antibiotic production
potential against human pathogens Pseudomonas aeruginosa (MTCC 741), Escherichia
coli (MTCC 1697), Staphylococcus aureus (MTCC 96) and Salmonella typhimurium
(MTCC 98). Out of nine bacterial isolates only MB-19 & MB-25 has shown significant
anti-microbial activities against Salmonella typhimurium and Escherichia coli
respectively. Potent endophytes were processed for identification of active metabolites at
Natural Product Lab, IIIM (CSIR) Jammu. Bacterial extracts were subjected to bioassay
for confirmation of presence of active metabolites in extracts. MB-19 & MB-25 has
retained antimicrobial activity. Methanol and chloroform extracts of MB-19 and MB-25
were subjected to HPLC. 13 peaks were recorded in chloroform extract and 11 peaks in
methanol extract for the culture.no. MB-19 and in MB-25, 18 peaks in chloroform extract
and 06 peaks of methanol extract were recorded. It is presumed that these peaks contain
active antimicrobial compound which can be identified further by NMR analysis of these
extracts. Our studies concluded that Arisaema erubescens retains ample biodiversity
within which might be the reason for its medicinal values. As indicated in our work
bacterial endophytes can be further exploited for industrial application.
Description
Keywords
HIMALAYAS, ARISAEMA ERUBESCENS, ENDOPHYTES AND PSEUDOMONAS AERUGINOSA