Heterosis breeding and protein profiling through sds-page in brinjal (Solanum melongena L.)
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Date
2009-03
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Present investigation was carried out during autumn winter season 2007-08 and 2008-09 at the Vegetable Research Centre of GBPUA&T, Pantnagar with 36 genotypes including 12 lines, 2 testers and 24 F1s of brinjal. The experiment was laid out in randomized block design with three replications. The observations were recorded for 12 characters viz. days to 50 % flowering, number of primary branches per plant, plant height (cm), fruit length (cm), fruit diameter (cm), number of marketable fruits per plant, weight of marketable fruits per plant (kg), number of unmarketable fruits per plant, weight of unmarketable fruits per plant (kg), total number of fruits per plant, total yield per plant (kg) and total yield (q/ha). The data were subjected to appropriate statistical analysis. Further, seed protein of 14 parents was utilized for sodium dodecayl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The analysis of variance revealed highly significant differences among genotypes for all the attributes under study. High heritability coupled with high genetic advance as per cent of mean was recorded for fruit length indicating importance of additive gene action controlling this character. The F1 cross PR × PS was best heterotic combination for total weight of fruits per plant and yield per hectare (q) over better parent. BARI × PS was best heterotic cross over mid parent and standard parent. Other combinations showing significant values for all types of heterosis were PB 69 × PS, Punjab Sadabahar × PU, PB 66 × PS, PB 64 × PS and PB 69 × PU. The findings of present investigation revealed that the parent PB 69 (plant height and yield), PB 67 (total number of fruits per plant, number of marketable fruits per plant and yield), SMB 115 (earliness, number of marketable fruits per plant and total number of fruits per plant), PB 66 (weight of marketable fruits per plant, number of marketable fruits per plant, total number of fruits per plant and yield), PB 64 (plant height, number of primary branches and fruit length), KS 331 (earliness, number of primary branches, plant height, fruit length), BARI (fruit length and earliness), Pant Rituraj (earliness and yield), Pant Samrat (total number of fruits per plant), PB 70 (earliness, total number of fruits per plant and yield), Pusa Upkar (earliness, plant height, weight of marketable fruits per plant and yield) were promising donor based on general combining ability. The cross BARI × PS showed highest economic heterosis and sca effects for most of the traits studied including the yield and yield attributing characters. Thus, this cross could be advanced to recover desirable segregants for the improvement of yield and yield contributing characters. The fourteen genotypes used as parents in present study could be distinguished based on sodium dodecyl sulphate polyacrylamide gel electrophoresis of seed proteins. They were distinguished into nine groups on different banding patterns in three zones (A, B and C). The UPGMA analysis showed that PB 60, PB 68 PB 64, Pant Rituraj, PB 66, BARI and PB 69, KS 331, PB 70, SMB 115, Punjab Sadabahar, Pant Samrat, Pusa Upkar formed two different clusters. However, PB 60; PB 68; PB 64 and PB 66; BARI and Pant Rituraj; KS 331, PB 69 and Punjab Sadabahar; PB 70, SMB 115 and PB 70; Pusa Upkar; and Pant Samrat were three different neighboring groups.
Description
Thesis-PhD
Keywords
heterosis, proteins, SDS-PAGE, aubergines, Solanum melongena , electrophoresis