K.V. Prasad
Loading...
Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
IARI, Discipline of Horticulture
Abstract
To establish a resourceful tissue culture system to induce anthocyanin pigments, it is necessary to
develop a reliable, rapid and efficient callus induction protocol. In this direction, research was conducted
to obtain high quality callus from leaf explants of Rosa hybrida cv. Pusa Ajay using different
concentrations of plant growth regulators (PGRs). For the establishment of aseptic cultures, a pretreatment
combination of 0.1 per cent carbendazim plus 0.1 per cent mancozeb-45 plus 200 mg l–1 8-
hydroxyquinoline citrate for 3 h followed by surface sterilization with 0.1 per cent mercuric chloride for
4 min was found to be the best owing to lowest microbial contamination percentage (7.33 ± 1.45) and
highest explant survival percentage (91.67 ± 1.20). Of the different treatments employed for callus
induction on Murashige and Skoog’s (MS) medium, a treatment combination of 19.60 μM indole-3-
butyric acid (IBA) plus 4.65 μM kinetin (Kin) plus 108.6 mM adenine sulphate (AdS) gave maximum
induction coefficient per cent (97.75 ± 1.11) and minimum days (5.75 ± 0.25) required for callus
initiation. Absolutely no callus induction was noted on the MS basal medium devoid of plant hormones
(control). The maximum gain in callus biomass accumulation, in terms of fresh- (382.00 + 1.47 mg) and
dry (14.40 + 0.05 mg) cell weight was recorded in the callus cultured on MS medium supplemented with
19.60 μM IBA plus 4.65 μM Kin plus 108.60 mM adenine sulphate. This treatment also resulted in the
lowest fresh- and dry cell weight ratio (26.52) suggesting actual gain in callus biomass accumulation.
Furthermore, correlation studies indicated a significant positive correlation between induction coefficient
and cell fresh- (r = 0.939) and dry- (r = 0.951) weight which implies that an increase in callus induction
coefficient in leaf explants will accompany an increase in cell fresh- and dry weights and their ratio.
Wounding of leaf explants was found to be beneficial for accelerating the callogenesis process. The
multiplication of callus was satisfactory on MS medium supplemented with doubled quantity of vitamins
and 19.60 μM IBA plus 4.65 μM Kin plus 108.60 mM AdS.
Description
T-8687
Keywords
anthocyanins, esters, planting, sucrose, productivity, biological development, tissue culture, biological phenomena, acidity, biosynthesis