Selection and characterization of chilli calli tolerant to fusarium wilt (Capsicum annuum L.)
Loading...
Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
The studies carried out on callogenic response of chilli species with different hormones revealed that MS medium supplemented with NAA 2.5 mg/1+ BAP 2.5 mg/1 was found most suitable for callus induction to both the genotypes viz., Capsicum annuum and Capsicum frutescens. The callogenic response to Fusarium oxysporum culture filtrate was more in C. frutescens (84.37%) than C. annuum (81.24%). There was no callus formation in both the genotypes when 20 per cent concentration of culture filtrate was used hence 15 per cent concentration of culture filtrate was used for in vitro screening for selection of tolerant calli. With the increase in concentration of culture filtrate, thereafter decrease in fresh as well as dry weight of calli derived from both the genotype was observed. Biochemical studies indicated that amount of total soluble sugars and reducing sugars were high in calli of both the genotypes that were grown on Fusarium oxysporum culture filtrate amended media. C. annuum showed decrease in non-reducing sugar content at 5 per cent concentration of culture filtrate whereas level of phenol and flavanol were high in both genotypes after selection. Activity of all the three enzymes viz., polyphenol oxidase, peroxidase and catalase increased with increase in concentration of culture filtrate compared to control.
Description
Keywords
Fungi, Enzymes, Concentrates, Chillies, Diseases, Planting, Fusarium oxysporum, Selection, Phenols, Biological phenomena