MOLECULAR CHARACTERIZATION OF GARLIC COMMON LATENT VIRUS (GarCLV), DEVELOPMENT OF IMMUNODIAGNOSTICS AND PRODUCTION OF VIRUS FREE GARLIC PLANTS THROUGH TISSUE CULTURE

Loading...
Thumbnail Image
Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
IARI,Division of Plant Pathology
Abstract
Twenty one garlic accessions grown on the research farm of the Indian Agricultural Research Institute, New Delhi and three local garlic lines from Kolar (Karnataka), Anand (Gujarat) and Pantnagar (Uttarakhand) were collected and tested for the presence of Garlic common latent virus (GarCLV). The occurrence of GarCLV was detected by direct antigen coated Enzyme linked immune sorbent assay (DAC-ELISA) performed with polyclonal antisera to GarCLV (Bioreba, Reinach, Switzerland) and flexuous filamentous particles were observed in immunosorbent electron microscopy (ISEM) after trapping with GarCLV specific antisera. Reverse Transcription polymerase chain reaction (RT- PCR) using a primer pair 5′-ATGTCARYGAGTGAAACAGA-3′ and 5′-CTAGTCTGCATTGTTGGATCC-3′ designed from the conserved regions of open reading frame 5 (ORF 5), which codes for coat protein (CP) of GarCLV, confirmed that all twenty four garlic accessions tested positive for GarCLV. Our study demonstrated wide spread association of GarCLV in garlic accessions including commercial variety, local farmer‟s cultivars and breeding lines. The potential impact of this infection on garlic production needs to be addressed.
Description
T-8669
Keywords
garlic, viruses, biological phenomena, planting, proteins, diseases, recombination, cloves, productivity, onions
Citation
Collections