Postvaccinal Immune Response to Regimens of Newcastle Disease Vaccination by Filter Paper Sampling Technique

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Date
1997
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Trop Anim Health Prod
Abstract
Seven hundred and ten blood samples were collected at random from commercial layers in Tamil Nadu on Whatman filter paper No. 1 instead of the conventional method of serum collection. The birds were subjected to different Newcastle diseae (ND) vaccinattion schedules and samples were collected to study the vaccinal response to ND at field level. Eluats were obtained from samples areas of filter paper using Brij-35 solution [detergent] and subjected to the micro haemagglutination inhibition (HI) test for ND antibodies. The HI titre ranged from less than 2^4 to 2^9. The possible causes of poor immune response to ND vaccinations are discussed.
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