Dot-enzyme linked immunosorbent Assay for Demonstration of Newcastle Disease Virus Infection
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Date
1999
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Comparative Immunology, Micobiology and Infectious Diseases
Abstract
Dot-enzyme linked immunosobent assay (ELISA) was standardised to detect Newcastle disease virus (NDV) specific antigen in chicken tissues, embryos and allantoic fluid samples. Samples positive by virus isolation were also found positive by haemagglutination (HA) and haemagglutination inhibition (HI) tests and by dot-ELISA but negative samples were found negative by all the serological tests used. Dot-ELISA was able to detect 0.25-0.50 HA units of virus. The emerging utility of dot-ELISA for diagnosis of Newcastle disease virus infection has been discussed . © 1998 Elsevier Science Ltd. All rights reserved.
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Keywords
Newcastle Disease Virus, Haemagglutination Test, Dot-ELISA, NDV Infection