Fixed Chicken Erythrocytes in Haemagglutination Test for Diagnosis of Newcastle Disease
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Date
1998-02
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Indian Vet. J.
Abstract
Haemagglutination (HA) of Newcastle disease virus (NDV) was first described by Burnet (1942) who found that this action to be inhibited by specific antiserum. HA of chicken erythrocytes (CRBC) and haemagglutination inhibition (HI) using specific antiserum is an accepted test for diagnosis of Newcastle disease (ND), since the test is sensitive, easy to perform and cost effective. HA and HI could also be used for field level diagnosis but preservation of CRBC is a main constraint. The present study was undertaken to increase the shelf life of CRBC by fixation using aldehydes for successful use of CRBC in HA and HI test in diagnosis of ND.