Demonstration of Newcastle Disease Virus-Specific Antigen by Fluorescent Antibody Technique and Immunoperoxidase Test
Loading...
Date
1997-11
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Journal of Animal Sciences
Abstract
Diagnosis of Newcastle disease (ND) in poultry depends on isolation and characterization of the virus (Alexander 1988). For diagnosis, it is sufficient to demonstrate that infection with Newcastle disease virus (NDV) has taken place and in this situation the infecting strain and its virulence are of no significance (Alexander 1988). Under such circumstances, fluorescent antibody technique (FAT) and immunoperoxidase test (IPT) were rapid for demonstration of virus-specific antigen in tissues (Maestrone and Coffin 1964, McNulty and Allan 1986, Lockaby 1993). This paper describes FAT and IPT for demonstration of NDV-specific antigen in impression smears and paraffin embedded tissue sections. FAT also used for screening blood smears and IPT for paraffin embedded chorioallantoic membrane (CAM) sections.
Description
Keywords
FAT, Newcastle disease diagnosis, IPT