Demonstration of Alternative and Classical Complement Pathway Activity in Colostrum from Buffalo (Bubalus Bubalis)
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Date
2003
Journal Title
Journal ISSN
Volume Title
Publisher
Veterinary Research Communications
Abstract
Buffalo colostrums caused lysis of unsensitized red blood cells (RBC) from sheep, goats, rabbits and chickens. RBC from cattle and buffalo were resistant to lysis. That lysis was due to the presence of natural antibodies to these RBC was ruled out since there was no reduction in haemolytic titres even after adsorption with the respective RBC. The addition of EGTA to the diluents had no effect on the haemolytic activity. These findings indicate the presence of alternative complement pathway (ACP) activity in buffalo colostrums. The haemoloytic activity of buffalo complement for unsensitized rabbit RBC was reduced to very low levels by heating at 50°C for 45 min. Treatment with zymosan also inhibited the haemolytic activity, while inulin had no effect. The maximum activity of ACP occurred in the presence of 4 mmol/L Mg^2+ in the diluent. The range of ACP activities in colostrums fro buffaloes varied from 4.06 to 8.48 CH50 units/ml. Using a standard system for titrating the classical complement pathway and rabbit red blood cells sensitized with goat haemolysin, the range of complement activity in buffalo colostrums was 4.81-6.77 CH50/ml.
Description
Keywords
Alternate Pathway, Buffalo, Classical Pathway, Colostrum, Complement, Heat Treatment, Haemolysin, Inulin, Magnesium, Zymosan