A Simplified Objective Method for Quantification of Peste Des Petits Ruminants Virus or Neutralizing Antibody
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Date
2000
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Virological Methods
Abstract
A simplified and standardized assay based on haemagglutination of infected culture supernatants was developed to detect peste des petits ruminants (PPR) virus growth in vero cells and to quantify PPR neutralizing antibody. Virus titres estimated by visual reading of cytopathic effects as a criterion were compared with those estimated by haemagglutination of infected supernatants and no statistically significant differences were seen between them. During quantification of PPR antibodies, the titres based on haemagglutination of supernatants on day 5 post-infection had a high sensitivity, specificity and agreement in qualititative comparison with those determined by visual examination of cytopathic effects. In quantitative comparison, the correlation coefficient between serum neutratlization titres estimated by visual examination of cytopathic effects or haemagglutination of supernatants was 0.96, when haemagglutination was done on day 5 post-infection. The virus and serum neutralization titres can thus be estimated objectively using the haemagglutination of supernatants as criterion to measure endpoints. The haemagglutination-inhibition titres also correlated well with serum neutralization titres with a coefficient of 0.78. Thus the haemagglutination-inhibition test appears to be a suitable alternative to the serum neutralization test for quantification of PPR neutralizing antibody. © 2000 Published by Elsevier Science B.V.
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Keywords
Serum Neutralization, Haemagglutination, Haemagglutination Inhibition, Cytopathic Effect