EVALUATION OF DIFFERENT POLLEN STORAGE METHODS IN SUNFLOWER (Helianthus annuus L.)

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Date
2009
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ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD
Abstract
The present investigation was taken up at Seed Research and Technology Centre, Rajendranagar, Hyderabad during rabi, 2008-09 to evaluate the effect of filler materials [(F1: fresh pollen without blending of filler material, F2: fresh pollen blending with borax @ 5% (75:25) and F3: fresh pollen blending with borax @ 5% + sucrose @ 5% (50:25:25)], pollen storage methods [(C1: moist earthen pot, C2: refrigerator and C3: earthen pot surrounded with moist sand] and age of pollen used for pollination (D0: same day, D1: one day, D2: two days, D3: three days, D4: four days and D5: five days)and their combinations on seed set (%), seed yield and seed quality of sunflower. The experiment was laid out in Factorial RBD with three replications. Fresh pollen without blending of filler material registered significantly more number of filled seeds plant-1, seed set (%), seed recovery (%), seed yield plant-1 (g), seedling dry weight (mg), field emergence (%), seedling vigour index-II. Pollen stored under refrigerator recorded higher seed yield plant-1 (g), filled seeds plant-1, seed recovery (%), pollen viability (%), germination (%) and seedling vigour index-I as compared to pollen stored in moist earthen pot. This may be attributed to low temperature of 4ÂșC and 75% RH prevailing in the refrigerator. The pollen stored in earthen pot surrounded with moist sand was adjudged as the next best treatment since the pollen viability could be maintained through in a lower scale up to fifth day. Irrespective of filler material and storage methods, the pollen viability (%), stigma receptivity, seed set (%) in terms of filled seeds plant-1, germination (%), seedling dry weight (mg), seedling vigour index-I and seedling vigour index-II were decreased with increased age of pollen. Field emergence (%), speed of emergence (%) was maximum with the same day and first day age of pollen used for pollination and subsequently decreased with increased age of pollen. Ethrel @ 25ppm, KNO3 @ 0.5%, GA3 @ 300ppm were shown better performance in respect of germination (%) compared to distilled water and control. Ethrel was found effective for breaking seed dormancy and gave above certification seed standards (70%). Correlation studies revealed significant negative relationship of seed set (%), seed yield plant-1 (g), pollen viability (%) and seed recovery (%) with the age of pollen indicating that as the age of pollen increases from 1st day to 5th day of storage seed set (%), seed yield plant-1 (g), pollen viability (%) and seed recovery (%) decreased.
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EVALUATION, DIFFERENT, POLLEN, STORAGE, METHODS, SUNFLOWER
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