Conventional and Molecular Approaches in Breeding for High Yield and Disease Resistance in Urdbean (Vigna mungo (L.) Hepper)

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Date
2011
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UAS Dharwad
Abstract
A study was conducted to understand the inheritance of resistance to powdery mildew and mungbean yellow mosaic virus (MYMV) in urdbean during 2008 to 2010. TAU-1, a high yielding but powdery mildew susceptible variety was crossed to LBG-17 which is resistant to powdery mildew. The F1, F2 and F3 populations were evaluated along with parents for resistance to powdery mildew under artificial condition. The study indicated that two independent dominant genes together control the resistance reaction in the host plant. Further, attempts were made to identify SSR markers inked to powdery mildew disease following the bulk segregants analysis in F2 population of this cross. Out of 469 SSR primers used for screening parental polymorphism, 32 primers could differentiate the two parents and two were found to be closely linked to powdery mildew disease resistance. Similarly inheritance of resistance to MYMV was studied by crossing TAU-1, (susceptible to MYMV disease) with BDU-4, a resistant genotype. The evaluation of F1, F2 and F3 and parental lines indicated the role of a dominant gene in governing the inheritance of resistance to MYMV. Attempts to identify the marker linked to MYMV did not give satisfactory result with 469 primers used for the study. Since TAU-1 is a high yielding cultivar, the F2 and F3 populations developed using this as one of the parents for inheritance study for diseases, were also evaluated for productivity and its component traits. F2 evaluation revealed high variability and also transgressive segregation. Similarly, evaluation of F3 families further based on selections made in F2 confirmed the superiority of few families in both the populations. Twelve families showing resistance to powdery mildew and high yield and nine F3 families showing resistance to MYMV and high yield were identified. It is suggested to carry forward these promising selections further to develop high yielding and disease resistant lines.
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