Detection of Rabies Virus Genes by In-Situ Polymerase Chain Reaction
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Date
2007-08
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Abstract
Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the āNā gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.
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Keywords
In situ PCR rabies diagnosis, Bovine serum albumin