Use of Multiplex PCR for Identification of Brucella Melitensis in Goats
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Date
2009-06
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Journal
Abstract
In this study, the use of multiplex PCR to detect B. melitensis directly in vaginal discharge and fetal stomach content using crude DNA preparation which did not require extensive sample preparation is reported. This assay has the advantage that the results can be determined immediately. This multiplex PCR will reduce the time, reagent cost required to diagnose brucellosis. However, more research is needed in direct analysis of clinical samples. Additional data are needed about the best choice of specimens and how long there is detectable DNA in those specimens over the course of infection.