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Theses - Gadvasu
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ThesisItem Open Access Semen characteristic & their correlations with testicular & body measurements in holstein friesian bulls(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 1981) Mohanty,Debendra NarayanThesisItem Open Access Process technology optimization for heat treatment of milk using solar concentrator(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2018) Jaglan, Neeraj; Dr. Amandeep SharmaThesisItem Open Access Prevalence of renal disease with special reference to glomerulonephritis in poultry(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2000) Sharma, Munish; Dr. N.K. SoodThesisItem Open Access Prevalence of bovine herpesvirus-1(BHV-1) infection in the semen of seropositive & seronegative bulls(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2002) Deka, Dipak; Dr. RamneekThesisItem Open Access Prevalence and molecular epidemiology of taenia solium cysticercosis in pigs in punjab(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2013) Chawhan, Pardeep; Dr. B.B. SinghThe present study was conducted to determine the prevalence and molecular characterization of porcine cysticercosis in Punjab. Among 519 pigs examined during post mortem inspection 22 were found positive showing the prevalence rate of 4.23 per cent. Age wise prevalence was more (5%) in pigs aged more than one year of age than those under one year of age (3.83%). Prevalence rate was found to be 5.6 per cent in males and 2.97 per cent in females. Cysticercus cellulosae infection had significant stastistical correlation between the infection and the breed and management practices. Three out of 519 examined pig carcasses, revealed the presence of cysts in their brains indicating the 0.57 per cent prevalence of neurocysticercosis. Morphological studies showed the typical characteristics of C. cellulosae i.e the presence of hooks in rostellum. Histopathological examination of tissues also aided in the diagnosis of the disease in pigs. In the present study, the product sizes of 286 bp, 420 bp, 1150 bp and 333 bp were obtained against the PCR amplification of large subunit rRNA gene , cytochrome oxidase I gene, internal transcribed spacer 1 gene and gene for the diagnostic antigen Ts14, respectively. All the samples were found positive for C. cellulosae using PCR.The large subunit rRNA gene primer and cytochrome oxidase I gene primer sets were found to be more sensitive (10 pg/µl) than internal transcribed spacer 1 gene primer (1ng/µl) for molecular detection of T. solium cysticercosis. Phylogenetic analysis based on the alignment of partial cytochrome oxidase-1 gene sequences indicated the presence of T. solium Asian genotype and absence of Latin American/African genotypes and T.asiatica from pigs in Punjab (India).ThesisItem Open Access Prevalence and molecular epidemiology of cryptosporidium parvum in cattle in punjab and its public health significance(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2013) Joutte, Jerry Ramnieng; Dr. J.P.S. GillThesisItem Open Access Prevalence and molecular charecterization of listeria species in bovine milk(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2013) Navsu, Sawant Laximan; Dr. Simranpreet KaurFood borne listeriosis has been recognized as an emerging bacterial disease of humans and animals worldwide. It is a serious invasive disease characterized by neural, visceral and reproductive clinical entities, leading to septicaemia, abortion, stillbirth, meningitis and meningoencephalitis, especially in immunocompromised individuals and persons in contact with animals. Milk and dairy products have been implicated as sources of listeriae in several widely publicized incidents, thus suggesting that the mammary glands of mastitic cattle may be an important reservoir of Listeria. A total of 539 samples (350 milk, 169 serum/blood and 20 hand swabs) were collected for prevalence and molecular characterization studies. The isolates were phenotypically and genotypically characterized by biochemical tests, haemolysis on sheep blood agar, CAMP test, PI-PLC assay and multiplex PCR targeting five virulence associated genes (prfA, plcA, actA, hlyA and iapA). A total of four pathogenic L. monocytogenes were recovered indicating prevalence rate of 1.14% in milk. All the four L. monocytogenes isolates were characterized as L. monocytogenes serotype 4b by multiplex PCR standardized for seotyping genes namely lmo0737, lmo1118, ORF2819, ORF2110 and prs. Antibiogram of the pathogenic L. monocytogenes isolates revealed sensitivity for amikacin, gentamycin, norfloxacin and doxycyclin. Animal sera (169) screened by indirect ELISA for antibodies against listeriolysin O showed sero positivity of 7.1%. Thus, the presence of pathogenic strains of L. monocytogens in raw milk appeared to be a cause for concern with serious public health implications.ThesisItem Open Access Postpartum ovarian activity in relation to calving season in buffaloes(bubalus bubalis)(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 1993) Kumar, Parveen; Dr. A.S. NandaThesisItem Open Access Polymerse chain reaction based assay for diagnosis of anaplasma marginale infection in tick infested cattle(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2013) Jaswal, Hitesh; Dr. Mandeep Singh BalPresent molecular diagnostic based study on Anaplasma marginale was designed keeping in view the importance of anaplasmosis in dairy cattle in the region. A total of 120 blood samples collected from tick infested dairy cattle from in and around Ludhiana district of Punjab state were examined by stained thin blood smear examination (STBS) for the presence of A. marginale infection in RBC’s. DNA extraction from blood and tick samples collected from animals in question was carried out by Hipura kit (Himedia) for blood and tissue respectively. The detection percentage by STBS was found to be 15.83%. Identification of ticks revealed more number of animals with Rhiphicephalus (Boophilus) microplus) infestation (89.16%) followed by Hyalomma anatolicum anatolicum (7.50%) and mixed infestation (3.33%). The BAP-2 and AL34S set of oligonucleotide primers used to amplify msp1β gene of A. marginale detected DNA in 39.16% (47/120) cattle blood. Detection percentage was more in adult cattle (42.30%) as compare to calves (18.75%). Study revealed over all detection percentage 26.67% (8/30) for A. marginale infection in tick samples. Detection percentage from ticks collected from adults was found to be higher (30% ; 6/20) as compare to calves (20%; 2/10). The semi nested PCR specific for Anaplasma genus revealed amplicon of 577 bp in 40.83 % (49/120) samples. The 577 bp product of semi-nested PCR for Anaplasma genus on digested with restriction endonuclease (Bst 1107I) at GTATAC site specific for A. marginale resulted into confirmed 85.72% (42/49) samples positive for A. marginale DNA and 7 (14.28%) remained undigested due to the absence of the restriction site indicating the presence of some other species of Anaplasma in the region. Necropsy findings from animal died of anaplasmosis revealed splenomegaly, hepatomegaly alongwith anemia and jaundice whereas histopathological findings revealed bile duct hyperplasia, bile retention, lungs emphysema, myocardial degeneration and fatty changes in liver.
