MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF COTTON [Gossypium hirsutum (L.)] HYBRIDS AND THEIR PARENTAL LINES
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Date
2012
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ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
Abstract
The present investigation was undertaken to characterize the cotton hybrids and
their parents using morphological and molecular markers. A field experiment was
conducted at college farm with randomized block design and laboratory studies were
carried out at Institute of Biotechnology, College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad. A set of 21 qualitative and quantitative morphological traits
and 16 Simple Sequence Repeats (SSRs) markers were used for the analysis. The study
was also intended to develop molecular ID fingerprints using SSR markers.
The leaf characters viz., leaf size, leaf shape, leaf colour, leaf incision, leaf
hairiness at peak flowering stage under field conditions were recorded. The leaf shape,
size and pubescence were useful in differentiating the cotton genotypes. Morphological
characters like growth habit, stem pigmentation, stem hairiness, plant height were
recorded. The growth habit and pubescence on stem could be easily identified and
categorized in to different groups.
The flowering character viz., days to 50 per cent flowering, petal colour, petal spot,
anther colour, stigma position, boll shape, bract type and number of serrations on bract
were useful in categorizing and differentiation of the cotton genotypes. The quantitative
characters like plant height, days to 50 percent flowering and days to maturity were
also useful for characterization of cotton genotypes as they exhibited variation.
DNA finger printing studies were useful to determine the hybridity and genetic
purity testing of cotton hybrids. About 16 most informative microsatellite markers were
employed for fingerprinting six cotton hybrids and their parental lines. These highly
informative primers not only differentiated the parent genotypes but also confirmed the
parentage of their true hybrids. Out of 16 primers only five microsatellite markers
differentiated the six hybrids and their parental lines. The SSR banding profiles of each
of the hybrid and their parents were observed manually for the presence or absence of
distinct bands. Comparison of the distinct banding patterns of the parents and their
progenies determined the true hybrids. The microsatellite marker, BNL 3449 amplified
alleles specific to differentiate parental lines of NSPHH 5 likewise BNL 3255 primer
for NDLHH 240 and WGHH 2, BNL 1317 primer for CSH 198, BNL 3090 primer for
CICR 2 and JESPR 148 primer for LAHH 4 . Thus the microsatellite banding pattern of
parents compared with its respective hybrids was clearly able to recognize the true
hybrid and the fingerprinting data were analyzed to create ‘Molecular IDs’ needed for
genotype characterization which was more informative and useful in identifying
genotypes.
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Keywords
planting, genetic processes, regeneration, pigeonpeas, genes, vegetative propagation, bacteria, biological development, cytokinins, biological phenomena