Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/66927
Authors: DIVYA, PEDADA
Advisor: KANAKA DURGA, K
Title: STUDIES ON CHARACTERIZATION OF GENOTYPES AND SEED STORABILITY AGAINST PULSE BEETLE (Callosobruchus chinensis) IN HORSE GRAM
Publisher: ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
Language: en
Type: Thesis
Series/Report no.: ;D9242
Agrotags: sowing, inorganic compounds, vegetables, grain legumes, biological interaction, developmental stages, storage, germinability, concentrates, sexual reproduction
GENOTYPES, SEED STORABILITY, PULSE BEETLE, Callosobruchus chinensis, HORSE GRAM
Abstract: “Studies on characterization of genotypes and seed storability against pulse beetle (Callosobruchus chinensis) in horse gram” were carried out under field conditions in National Bureau of Plant Genetic Resources (NBPGR), Regional Station, Hyderabad and the seed storability studies under laboratory conditions in Seed Entomology Lab, Seed Research and Technology Centre, ANGRAU, Rajendranagar, Hyderabad, Andhra Pradesh during the year 2011-12 Fifty horse gram accessions were characterized based on the morphological characters viz. plant growth pattern, plant growth habit, stem colour, leaf colour, seed colour. Significant correlations were noticed among the qualitative traits while biochemical parameters exhibited positive and negative relationship with the morphological qualitative traits but the relationship is non-significant. Plant growth pattern is significantly and positively correlated with plant growth habit and leaf colour while a significant negative relationship was noticed between plant growth pattern and stem colour. Plant growth habit showed significant negative relationship with stem colour and significant positive relationship with leaf colour. However, biochemical parameters viz., proteins and phenols exhibited positive and negative relationship with the morphological qualitative traits but the relationship is non-significant. The performance of 50 horse gram accessions against C. chinensis infestation was assessed based on the development of the insect, per cent weight loss and insect damage. Significant variation was observed among the accessions with respect to pest development and seed damage. Based on the biological parameters of the pulse beetle viz., oviposition, adult emergence, mean developmental period, per cent insect infestation and weight loss, Palem-2, Palem-1, AK-21 and NSB-27 were considered to be resistant accessions. On the other hand, NS/05/42 and NSJ/NAIP/BD-ADB-35-1 were found to be susceptible accessions. The per cent reduction in germination in comparison to initial germination is 0.4%, 2.4% and 2.4% for Palem-1, Palem-2 and AK-21, respectively at 60 days after release whereas in NS/05/42, the per cent germination drastically reduced from 90.6 to 31.6% leading to 65% reduction in germination after infestation. On the basis of Howe susceptibility index, the accessions Palem-2, KSAS/06/391, Palem-1, AK-21, NSB-27, NSJ/NAIP/140-239 and NDS-259 were categorized as resistant accessions, while NS-74, RJR-94, PSRJ-13089, NS/05/113, NSM-125 and PSRJ-13089-1 were grouped as moderately resistant accessions. NSJ/NAIP/031-130, BAR-231-1, NSJ/NAIP/006-105, PSRJ-13030, NS/05/94 and NS/05/87 were classified as highly susceptible accessions to bruchid infestation. Among the biochemical and physico-chemical characters, proteins, phenol content and seed coat thickness of horse gram accessions showed significant influence on oviposition, developmental period, adult emergence and seed infestation of C. chinensis. Less protein content, high phenols and thick seed coat of the accessions were detrimental to the growth and development of C. chinensis while high protein content, low phenols and thin seed coat of the test accessions favoured the successful development of bruchids of pulse beetle. Management of pulse beetle through modified atmosphere using elevated levels of CO2 indicated that 40% and higher concentrations of CO2 significantly reduced the per cent insect infestation and weight loss of horse gram seed. The efficacy of different concentrations of CO2 on seed infestation and population build up revealed that exposing the bruchid infested horse gram seed to 40% and higher concentrations of CO2 not only checked seed infestation but also checked the progeny production of the pest even after prolonged periods of storage up to 6 months. The horse gram seed stored in CO2 rich atmosphere also maintained seed quality with high germination% and seedling vigour up to six months of storage.
Subject: Seed Science and Technology
These Type: M.SC
Issue Date: 2012
Appears in Collections:Theses

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