Fertility In Bitches Following Intravaginal And Intrauterine Insemination With Fresh And Frozen Semen

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Date
2007
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Tamil Nadu Veterinary and Animal Sciences University
Abstract
This study was conducted to compare the conception rate and litter size in bitches inseminated following intravaginal and intrauterine route with fresh and frozen semen and to study the effect of commercial semen extender on post thaw characters of dog semen. To study the efficacy of vaginoscopy to predict fertile insemination time in bitches was also taken as one of the objectives. Sixty bitches were randomly divided into six groups viz., Group I, II, III, IV, V and VI 10 each. The time of insemination was based on vaginoscopy in group I to V and on vaginal exfoliative cytology in group VI bitches. Semen was collected from seven male dogs of different breeds, evaluated and used for cryopreservation either with Tris or Tes-tris extender.Group I and II bitches were subjected to intravaginal insemination while Group III and IV bitches were subjected to intrauterine insemination using fresh and frozen semen, respectively. Group V and VI bitches were inseminated intravaginally using frozen semen supplemented with prostatic fluid and with fresh semen using vaginal exfoliative cytology to determine time of insemination, respectively. The conception rate obtained in this study was 50, 20, 50, 30, 30 and 40 per cent in group I, II, III, IV, V and VI bitches respectively. The mean post thaw motility was significantly higher for semen cryopreserved with Tris when compared to Tes-tris extender. The percentage of mean abnormality was significantly lower in semen cryopreserved with Tris when compared to Tes-tris extender. The mean percentage of plasma membrane integrity was significantly higher for Tris when compared to Tes- tris extender. Hence, it is concluded from this study that the conception rates were similar for both intravaginal and intrauterine insemination with fresh semen and higher conception rates were obtained when frozen semen was deposited intrauterine rather than intravaginal. Semen cryopreserved with Tris extender was found to gave better post thaw characters compared to Tes-tris extender. Vaginoscopy was found to be effective in assessing the optimum insemination time compared to vaginal exfoliative cytology. Addition of prostatic fluid with frozen semen had significant influence on increasing the conception rate with intravaginal insemination.
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Keywords
Fertility, Artificial insemination, Bitches
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