Development Of BHK 21 Cell Culture Adapted Peste Des Petits Ruminants (PPR) Virus Vaccine
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Date
2006
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Publisher
Tamil Nadu Veterinary and Animal Sciences University
Abstract
The present study was undertaken to develop a BHK 21 cell culture
adapted Peste des petits ruminants (PPR) virus vaccine. The PPR
vaccine virus AR 87 adapted in Vero cell line monolayer was propagated
in BHK 21 cell line monolayer and BHK 21 suspension cell line (Razi,
Indian Immounological, Hyderabad) by giving fifteen and five serial
passages respectively. The identification of the PPR vaccine virus
adapted in BHK 21 cell line monolayer and BHK 21 suspension cell line
carried out by HA and VNT and further confirmed with S-ELISA. The
peak titre of PPR vaccine virus in BHK 21 cell line monolayer at 5 th , 10 th ,
15 th and 1 st , 2 nd , 3 rd , 4 th and 5 th passage levels in BHK 21 suspension cell
line at 72 hrs post infection were 8.23 log 10 , 8.41 log 10 , 8.54 log 10 , 9.22
log 10 , 9.31 log 10 , 9.50 log 10 , 9.66 log 10 and 9.82 log 10 TCID 50 / 0.1ml
respectively. The standardization of the PPR vaccine virus adapted in
BHK 21 suspension cell line were carried out by production of master seed
virus, production of working seed virus, purity test, safety test and
potency test. The PPR vaccine virus AR 87 adapted in BHK 21 suspension
cell line was tested for potency and efficacy in sheep and goats. All thevaccinated animals were screened for seroconversion by HI, SNT and C-
ELISA and produced sero conversion.
The field and comparative study of both the PPR vaccine virus
AR 87 adapted in BHK 21 suspension cell line and PPR vaccine virus AR
87 adapted in vero cell line monolayer were tested in sheep and goats.
Serum samples were collected on day 0 and 21 st day post vaccination and
screened for seroconversion by HI, SNT and C–ELISA and the titre
compared between the two vaccines. The PPR vaccine developed in
BHK 21 suspension cell line was found to give a good antibody response
compared to PPR vaccine developed in vero cell line.
Description
Keywords
PPR, HA, VNT, HI, SNT, S-ELISA, C-ELISA