Isolation and Characterization of Pgprs from Rhizosphere of Banana Against Tip Over Disease Caused by Erwinia carotovora subsp. carotovora
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Date
2018-08
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University of Agricultural Sciences, Dharwad
Abstract
Survey conducted during 2016-17 revealed that the incidence of tip over disease of banana was ranged from 1.00 to 48.00 per cent with highest per cent mean disease incidence in the Belagavi district (14.41 %) and lowest in Dharwad district (6.06 %). Mean maximum disease incidence 12.55 per cent and 13.88 per cent was observed in black soil and Grand-naine variety respectively and total of 134 isolates of plant growth promoting rhizobacterias (PGPRs) were isolated from the rhizospheric soil of banana collected. Highest numbers of isolates were from Bagalkote district (41) and least from Dharwad district (8). Among them 64 isolates were found to be as Bacillus spp. And remaining 70 isolates were found to be as Pseudomonas spp.
The ability of isolated PGPRs to suppress the growth of Erwinia carotovora subsp. carotovora was studied in vitro. Among 12 effective isolates of Bacillus spp., Belagavi isolate 13 has recorded maximum inhibition of 16.67 mm and least inhibition (12.00 mm) was observed by Haveri isolate 5. Among 7 effective isolates of Pseudomonas spp., maximum inhibition (18.00 mm) was observed by Belagavi isolate 8 and minimum inhibition (12.00 mm) was observed by Dharwad isolate 2.
Molecular characterization of pathogen was identified as Erwinia carotovora subsp. carotovora. Biochemical and molecular characterization of effective isolates of PGPRs were identified as Bacillus cereus (Belagavi isolates 12, 13 and Vijayapur isolate 2), Bacillus subtilis (Vijayapur isolates 4, 7, Bagalkote isolate 15 and Haveri isolate 5), Bacillus pumilis (Vijayapur isolate 8), Pseudomonas aeruginosa (Belagavi isolates 4, 8, 9, Haveri isolate 3, and Vijayapur isolate 3) and Pseudomonas putida (Bagalkote isolate 5). Under in vitro maximum inhibition (39.00 mm) and (25.00 mm) was observed by Silvax® and K- cycline at 500 ppm and 750 ppm respectively.