Fertilization Barriers In Interspecific Hybridization Between Cucurbita Pepo L. And Cucurbita Moschata Duchesne
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Date
2023
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Punjab Agricultural University
Abstract
The hybridization barriers in Cucurbita moschata (Punjab Nawab, Punjab Sardar) and Cucurbita pepo (PMK-1) genotypes were investigated in the present study. The results revealed that the pollen grains were viable and stigmas were receptive at the time of pollination in all the genotypes. The maximum pollen germination (%) was observed in Punjab Nawab, while maximum viable pollen size, pollen tube length and longest style length was recorded in Punjab Sardar. Since, the shorter pollen tubes of PMK-1 did not grow enough in the longer style of Punjab Sardar, hence the interspecific cross of Punjab Sardar ×PMK-1 was incompatible. While in the reciprocal cross (PMK-1×Punjab Sardar) formation of callose plugs in the pollen tubes of Punjab Sardar and its slow growth might had contributed to the failure of fertilization. However, in the interspecific cross of Punjab Nawab × PMK-1 and vice-versa the pollen grains successfully germinated and pollen tubes reached the style, hence the cross was partially incompatible but embryo was not able to attain a particular shape. The fruit setting (%), fruit diameter, fruit weight, seed number, seed weight and seed germination (%) was lesser in interspecific crosses as compared to self-pollinated genotypes. Further, cluster analysis revealed that partially compatible and incompatible crosses are present in different clusters and partially compatible cross showed strong positive correlation with fruit characters. The information of fertilization barriers would be helpful for the breeders to overcome these barriers and introgress the hull-less seed trait of C. pepo and diseases resistant trait of C. moschata.
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Kapoor, Ishita (2023). Fertilization Barriers In Interspecific Hybridization Between Cucurbita Pepo L. And Cucurbita Moschata Duchesne (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.