STUDIES ON INHERITANCE OF SEED COAT COLOUR AND MOLECULAR MAPPING OF Fe AND Zn IN MNK-1 × A-1 DERIVED F2:3 POPULATION IN CHICKPEA (Cicer arietinum L).
Loading...
Date
2023-12-29
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Bio-fortification through plant breeding is a cost-effective and sustainable approach
toward addressing micronutrient malnutrition prevailing across the globe. With the aim of
identifying the genomic regions controlling seed Fe and Zn concentrations, the F2:3
population derived from a cross between MNK-1 and Annigeri-1 was genotyped using GBS
approach and evaluated for Fe and Zn at ICRISAT, Hyderabad. An intraspecific genetic
linkage map comprising 839 SNPs spanning a total distance of 1,088.04 cM with an average
marker density of 1.30 cM was constructed. 11 QTLs were identified for seed Fe on CaLG03,
CaLG04, and CaLG05, with phenotypic variation explained ranging from 7.2% (CaqFe3.4)
to 13.4% (CaqFe4.2). For seed Zn, eight QTLs were identified on CaLG04, CaLG05, and
CaLG08. The QTLs individually explained phenotypic variations ranging between 5.7%
(CaqZn8.1) and13.7% (CaqZn4.1). Three QTLs for seed Fe and Zn (CaqFe4.4, CaqFe4.5and
CaqZn4.1) were co-located in the “QTL-hotspot” region on CaLG04 that harbors several
drought tolerance-related QTLs. These genomic regions and the associated markers can be
used in marker-assisted selection to increase seed Fe and Zn in agronomically superior
chickpea varieties. Variability studies revealed, there is significant amount of variability for
quantitative traits in F2:3 population in the cross MNK-1 × A-1 and F2 in MNK-1 × GKB-10
cross. Genetic parameters such as PCV and GCV were high for traits viz., number of pods per
plant, number of seeds per plant, seed yield per plant and iron content of seed. High
heritability coupled with high GAM was observed for number of pods per plant (91.20% and
72.06%, number of seeds per plant (98.01% and 72.36%), seed yield per plant (89.97 % and
67.00%), hundred seed weight (82.62% and 31.95%), Fe (97.62% and 44.44%), Zn (89.82%
and 28.99%) content and plant height (80.35% and 23.82%). Inheritance of seed coat color
followed the trigenic mendelian ratio 27:9:9:9:3:3:3:1 and flower colour 3:1 ratio
Antinutritional factors in parents are under the permissible limit. Hence we can expect elite
high yielding lines with high iron, zinc and low antinutritional factors as correlation between
yield and iron and zinc is non-significant negative association.