Evaluation of low-cost media for Azospirillum sp. based biofertilizer production
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Date
2023
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Punjab Agricultural University
Abstract
Different media formulations of wheat bran and vegetable waste were evaluated for growth of Azospirillum sp. in terms of optical density and logCFU/ml. The optical density (OD) enhanced gradually from 0 to 3rd day followed by constant values onwards. Maximum optical density was recorded in the standard broth i.e., (0.67±0.02)where among the low-cost bioformulations, maximum OD was observed in 40% of wheat bran (WB) (0.67±0.01) followed by 50% of WB. Observations revealed that wheat bran supported comparably better growth tan vegetable waste.The viability of Azospirillum sp.biofertlizer culture wasthen assessed on the 3rd day of incubation wherestandard broth i.e., 9.28±0.02log CFU/mlhad the highest viable cell density compared to 40% WB exhibiting (8.44±0.02log CFU/ml)growth for Azospirillum sp.Analysis of different liquid media formulations for indole acetic acid production, ammonia production, phosphate and zinc solubilization revealed that 40% WB followed by 50% WB were found to be the foremost media formulations where among the vegetable waste (VW) concentrations, 30% of VW exhibited the highest values, but were comparatively lower than that of the WB formulations.On the basis growth and multifunctional traits, the treatments were narrowed down to 50% and 40% of WB and 30% of VW and Jensen’s broth was taken standard broth. Assessment for the seedling emergence, shoot and root length of rice (PR1121) under axenic conditions showed 40% WB to be the most prominent media formulationamong low cost formulations.Additionally, the viable population of Azospirillum sp. biofertilizer cultures was also monitored at monthly interval where gradual decline in the viable cell countof Azospirillum sp. in all the treatments was observed when standard broth was found to be desirable at one month interval followed by sudden drop in the population. It can be deciphered that 50% and 40% of wheat bran can be used as a potential liquid media formulation to develop a low-cost liquid Azospirillum sp.
based biofertilizer on the condition of its optimization to enhance the growth and shelf life.
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Jaspreet Kaur (2023). Evaluation of low-cost media for Azospirillum sp. based biofertilizer production (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.