Evaluation of Plant Growth Promoting Rhizobacteria (PGPR) and Nano zinc in maize (Zea mays) crop
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Date
2022
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Department of Soil Science & Agricultural Chemistry, BAU, Sabour
Abstract
An experiment was conducted in Bihar Agricultural University involving isolation, characterization, identification and analysis of the plant growth promoting activities of 10 bacterial strains followed by a 2-year field trial of selected PGPR isolates on Maize crop involving evaluation of 2 PGPR strains along with ZnO-NP in Maize crop to study the impact of biofertilizer and nanofertilizer on growth and yield of Maize. Isolates M6- Bacillus aryabhattai and M9-Enterobacter cloacae strain BAU3 were found to be the most promising strains and were proved to be efficient PGPR strains. At different intervals i.e. 5, 10, 15, 20 days after inoculation strain M9 could fix nitrogen (13.50, 3.70, 3.86, 4.24 mg/l), solubilize phosphorus (13.50, 13.70, 13.86, 13.97 mg/l), potassium (3.30, 3.80, 4.29 mg/l) and could produce GA with excellent pH reduction and cell growth (OD). Strain M6 was also able to fix nitrogen, solubilize phosphorus (10.06, 9.40, 13.20, 13.79 mg/l), potassium (2.90, 3.50, 3.77 mg/l) and could produce IAA significantly and higher than other strains with good reduction in pH and cell growth. Foliar spray and seed treatment of isolates Bacillus aryabhattai strain M6 (Accession No. MN533949), Enterobacter cloacae strain M9 BAU3 (Accession No. MK033472) and spray of nano-zinc increased plant height at 75 DAS (95.71 cm), chlorophyll content at 75 DAS (83.14), LAI at 75 DAS (1.89), FW at harvesting (70.87 g/plant), DW at harvesting (21.37 g/plant) of shoot and FW at harvesting (17.42 g/plant), DW at harvesting (3.01 g/plant) of roots. PGPR inoculation and ZnO-NP was also responsible to increase Seed index (29.35g), cob weight (0.2505 kg), No. of cobs/plant (4.34) and yield (54.67 q/ha). The same treatment combination increased available N (212.05 kg/ha), P (20.53 kg/ha), K (160.25 kg/ha), Zn (1.34 ppm), Fe (37.81 ppm), Mn (26.25 ppm), Cu (3.20 ppm) in post-harvest soil along with the uptake of nutrients in grains as well as total plant. Different forms of Phosphorus and Zinc also increased in this particular treatment along with the biological properties of soil under the influence of the combination of 2 PGPR isolates and ZnO-NP.