Analysis and Characterization of Heat Stress Gene/s in Wheat (Triticum aestivum L.) Genotypes
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Date
2021
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SARDARVALLABH BHAI PATEL UNIVERSITY OF AGRICULTURE MEERUT 250110
Abstract
During the few decades, the global climate has changed much; the effect of
increasing temperature on wheat production is acquisition global concern. Heat is the
foremost abiotic stress limiting the wheat production. Wheat yield rate of the world is
reduced as much as 15% by the high-temperature stress. Heat stress interferes the
essential physiological and biochemical parameters of the crops. In stress condition
Plant rapidly produces heat shock proteins to reduce the effect of heat stress. Heat
stress affected all the genotypes of wheat under treatment (37º C) for 12h.
Physiological Parameter (Chlorophyll content Photosynthetic rate, Relative Water
Content, and Injury % in Cellular Membrane Stability) was observed to be decreased
in all genotypes and Biochemical parameter (Proline Content, Catalase and
Peroxidase) was observed to be increased under heat treatment condition. In
Physiological parameters, DBW16 WH157 and RAJ3765 genotypes were found the
most tolerant. Regarding biochemical parameters, DBW16, RAJ3765, WH157,
PBW373, and WH730 were most tolerant among under heat stress condition. The
selected nucleotide sequence of HSP90, CAT, and TapAPX were amplified using
PCR. The sequences of amplified products were as 320bp, 395bp, and 195bp
respectively isolated by DBW16, RAJ3765 and WH730 genotypes. These three genes
HSP90, CAT and TapAPX were characterized by in-silico analysis. On the basis of
physiological biochemical and in-silico molecular analysis six genotypes were
selected for functional analysis. The HSP90 gene showed highest level of transcription
fold change 5.10 and 5.05, which increased during treatments of 4h at 37°C in
genotypes DBW71 and RAJ3765. The CAT gene also showed highest level of
transcription fold expression 13.5 and 13.3, which increased during treatments of 6h at
37°C in genotypes DBW16 and RAJ3765. The TapAPX gene showed highest level of
fold change 14.8, which increased during treatments of 6h at 37°C only in genotype
RAJ3765. Out of overall analysis genotypes DBW71, DBW16, RAJ3765, PBW373,
WH730 and WH157 studied genotypes were found the most tolerant. All genes
(HSP90 CAT and TapAPX) were associated with resistance to heat stress showed the
constitutive higher expression. Therefore, these could be implemented into the crop
improvement programs for heat tolerant and further characterize in other important
crops to better understanding of its expression behavior in different stress condition
Description
Analysis and Characterization of Heat Stress Gene/s in
Wheat (Triticum aestivum L.) Genotypes
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Citation
Analysis and Characterization of Heat Stress Gene/s in Wheat (Triticum aestivum L.) Genotypes