INTROGRESSION STUDIES OF FUSARIUM WILT RESISTANCE IN PKV KABULI 4 USING MOLECULAR MARKERS.
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Date
2021-10-31
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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
The present investigation entitled “Introgression studies of Fusarium wilt resistance in PKV Kabuli 4 using molecular markers” was carried out at the Biotechnology Center and Pulses Research Unit, Department of Agricultural Botany, Dr. PDKV, Akola.
The MABC program was initiated with the cross between PKV 4 (Moderately susceptible, recurrent parent) and WR-315 (Resistant, donor parent) to develop Fusarium wilt resistance to foc-1 during crop season September 20018-19. Total 67 different molecular markers were used for the parental polymorphism survey and seven markers found polymorphic namely, TA 194, TS 82, TA 59, TA 110, showed 100 % polymorphism and TR 19, STMS 02, TA 03 showed 75 % polymorphism. The identified polymorphic markers help in differentiating male and female parental lines and successfully detect the F1 hybrid progenies. From the hybridization program 75 F0 were seeds obtain Among 75 F1s plants, 45 hybrids were confirmed through using polymorphic markers (TA 14, TA 194, and TR 19). Further, the confirmed hybrids were used for the execution of the first round of backcrossing and 18 BC1F1 plants were grown at this point foreground and recurrent parent genome recovery was carried out.
Foreground selections were carried out using linked markers namely, TA 59, TA 110, TA 194, TR 19 for selection of introgressed target region and six common heterozygous plants were selected. In BC1F1 population selection for target, the region was carried out using the linked markers.
For identification of Fusarium wilt QTLs in the F2 population, 60 F2 individuals from a single cross were used simultaneously for genotyping by six polymorphic markers and phenotyping in the artificial greenhouse sick pot conditions. Using morphological and molecular data linkage map was constructed and one major QTL “qfwch-02” were identified. Which covered a total map length of 93.60 cM, the average map distance between any pair of markers was 16.1 cM and major QTL “qfwCh-02” was identified on linkage group 2 for Fusarium wilt resistance against race-1 at map position 31.4 cM in F2 progenies of PKV Kabuli 4 X WR-315. This QTL explained 10.91 % phenotypic variance with LOD score 3.3 flanked by left marker TA 110 and right marker TR 19.
Background selection was performed in 18 BC1F1 individuals using 20 molecular markers distributed throughout the chickpea genome. The range of RPGR analysis in BC1F1 was ranged from 57 % (BC1F1-180) to 67.5 % (BC1F1-17).
During the present investigation the validated markers, for foreground selection namely, TA 100, TR 19, TA 194, TA 59 for foc-1 race will help in early detection of resistant and susceptible plants in chickpea mapping population and to introgress fusarium wilt resistance in chickpea genotypes by Marker Assisted backcross breeding program.
Developed genomic linkage map and QTLs identified will be useful for chickpea genetics and breeding applications. Moreover, linked markers found with identified QTLs for FW race-1 will be useful for molecular breeding for Fusarium wilt chickpea improvement.
Description
All molecular experiments were conducted at the Biotechnology Centre, Department of Agricultural Botany, Dr PDKV., Akola. Field experiments related to plant breeding were carried out at Pulses Research Unit and on the experimental field of Biotechnology Centre, Dr. PDKV., Akola. All experiments were carried out during the Rabi season year of 2018 to 2021. Hybridization programs were executed for the development of mapping population and molecular breeding work was carried out at the molecular breeding laboratory at Biotechnology Centre, Department of Agril. Botany. Dr. PDKV., Akola.
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Citation
KHELURKAR, VAIBHAV CHANDRAKANT. (2021). Introgression studies of Fusarium wilt resistance in PKV Kabuli 4 using molecular markers. Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2021. Print. X, 127p. (Unpublished).