A study on retinoic acid inducible gene1 like receptors (RLR) mediated in-vitro immune response in goats
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Date
2022-08-18
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Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (J&K)
Abstract
The aim of this work was to study the in vitro innate immune response in goats through retinoic acid inducible gene-1 like receptors (RLR). The study was conducted on Bakarwal and indigenous non-descript goat, both native to Jammu and Kashmir Union Territory, and immune response variation through RLR was studied in both goat types. Primary culture of foetal fibroblast cell of Bakarwal and non-descript goat was established through explant technique. The study also investigated the use of Poly I:C in simulating dsRNA infection in the cell culture, and the successful induction of RLR mediated antiviral response in both goat foetal fibroblast cell types. The expression profiles of the entire RLR pathway were evaluated through mRNA quantification employing qRT-PCR technique. The following host pathway components- RLR (RIG1, MDA5), adaptor molecules (MAVS, IRF3, IRF7) and effector molecules (ISG15, ISG54 and ISG56) were evaluated. The intensity of antiviral response induced by poly I:C was maximal after 4 h post transfection. A wavy pattern of expression of RIG1 and MDA5 was observed for both the goat cell types. Respone was early but of lesser duration in non-descript goat cells, but late and of intense type in Bakarwal breed. It may be concluded that the pattern of antiviral response induced by RLRs after stimulation could vary between goat breeds. Sequence analysis of RIG1 and MDA5 genes revealed conservation of critical amino acid residues in goats.