Clinico-pathomorphological changes and their amelioration by Alstonia scholaris in experimentally induced Salmonella Gallinarum infection in broiler chickens
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Date
2022-05-17
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Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (J&K)
Abstract
The present study was carried out to evaluate the effect of Alstonia scholaris in ameliorating clinico-pathomorphological changes in experimentally induced Salmonella Gallinarum. A total of 120, one day old birds were allocated to four groups with 30 birds each. Group I served as control. Extract of A. scholaris was administered @ 200 mg/L in water to group III and IV birds via drinking water. At three weeks of age birds in group II and IV were challenged with S. Gallinarum 2 X 109 CFU. Sequential sacrifice of the 6 birds from each group was carried out at 3, 6, 9, 12, and 15 days post infection. Blood was collected for hematological, biochemical, oxidative stress studies and after sacrifice samples from liver, kidney, brain, heart, lung, spleen were collected for histopathological studies. Mortality was 43.33% in group II and 20% in group IV. Significant decrease in the values of haemoglobin, packed cell volume and total erythrocyte count and increase in total leucocyte count and percent heterophils values were, recorded in group II birds. Significant alterations in oxidative stress parameters were seen in liver, kidney, intestine and blood in group II. The values of total protein, albumin and alkaline phosphatase were lower but that of globulin, aspartate transaminase, alanine transaminase, creatinine, blood urea nitrogen were higher in group II as compared to group I. Enlargement, mottling, multifocal necrosis and congestion with heterophilic infiltration in liver and spleen along with congestion, hemorrhages, necrosis and inflammation of intestine, brain, kidney, heart and lungs were recorded in Salmonella infected birds. Administration of A. scholaris extract significantly ameliorated changes in hematobiochemical indices, oxidative stress parameters and gross as well as histological in Salmonella infected group.