Title: IN-VITRO PROPAGATION OF JAMUN (Syzygium cumini L.).
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Date
2022-10-26
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Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Abstract: The present study was carried out with the aim to study an in-vitro propagation of Jamun (Syzygium cumini L.) species using local species using Lloyd and McCown woody plant medium (WPM) in CRD experimental design at Biotech Centre, Dr. PDKV, Akola during 2019-20 and 20-21. Effect of WPM with little modification of cytokines, auxins, and plant growth-stimulating hormones on different growth parameters on in-vitro propagation were optimized. Surface sterilized local jamun nodal explant using HgCl2, the combination of antibiotics and antioxidants were attempted. Surface sterilization of the explants was effectively achieved using mercuric chloride at 0.1% (4 minutes) in combination with 1% streptomycin as it recorded lower contamination (20%) and higher survival (76.66%). Surface sterilized nodal explant using HgCl2, the combination of antibiotics and antioxidants were attempted using WPM with BAP (6-Benzylamino purine), Kn (kinetin) shown good results. Nodal explants were cultured on WPM supplemented with BAP (6-Benzyl amino purine 8.0 and 9.0 µM/l), Kn (kinetin 8, 8.5 and 9 µM/l). In which, WPM with BAP + Kn @ 8 µM/l was proved to be significantly superior at 1% level for high survival rate after 3rd week 76.66% and requires less time 25.59 days to shoot sprout. Whereas BAP + Kn @ 8.0 + 8.5 µM/l was proved best with the highest 2.97 shoot multiples. Shoot proliferation and multiplication were performed on WPM supplemented with BAP @ 13 and 14 µM/l, Kinetin @ 8.0, 8.5, and 9.0 µM/l, and NAA (Naphthalene acetic acid) @ 5 µM/l. The treatment combination of WPM with BAP + Kn + NAA @ 13+8.5+5.0 µM/l were proved to be significantly superior at 1% for high frequency multiple shoot induction. The response was shown a maximum of 5.66 shoot multiples after 2nd subculture, followed by 3.94 shoot multiples after 1st subculture with significantly highest 1.98cm shoot length elongation upon WPM with 13.0+ 8.5+5.0 µM/l BAP + Kn + NAA medium as against 0.57cm of control (WPM without growth regulators). Therefore, woody plant media with above modification found promising for in-vitro propagation of Jamun species.
Description
Description: The present investigation was conducted at Plant Tissue Culture Laboratory, Biotechnology Centre, Dr.P.D.K.V., Akola (MH). The present study is designed to standardize the effect of woody plant medium along with particular growth regulators for in-vitro proliferation of Jamun through nodal segment. In-vitro technique ensures continuous supply of planting material to fulfill the medicinal needs also conserves the germplasm by producing genetically identical plant.
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Citation
Citation: Bochare, Prachi Rajendra. (2021). In-vitro propagation of Jamun (Syzygium cumini L.). Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2021. Print. xiii, 67p. (unpublished).