Genetic characterization of indigenous goats of Keonjhar district of Odisha
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Date
2021
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Department of Animal Breeding and Genetics, OUAT, Bhubaneswar
Abstract
Data on different conformation, production and reproduction traits were collected
from 120 indigenous Keonjhar goats after collecting weights and measurements of the body.
The data that correspond were also collected from 120 Black Bengal and 120 Ganjam goats
for comparison. The mean values of weight at birth in male Keonjhar, Black Bengal and
Ganjam goats were 1.36±0.20, 1.41±0.20 and 2.68±0.22 kg, respectively. Correspondingf
mean valuessin female goatsswere 1.28±0.22, 1.30±0.21 and 2.55±0.22 kg, respectively. At
adult stage, the male Keonjhar, Black Bengal and Ganjam goats achieved 15.85±0.21,
24.40±0.23 and 41.39±0.20 kg of body weight, respectively. The values that correspond in
case of female goats were found to be 14.90 ± 0.23, 22.31±0. 88 and 30.31±0.52 kg,
respectively. All the body morphometric traits were positively correlated among each other in
this study. Females of Keonjhar, Black Bengal and Ganjam goats came to heat at
229.18±1.88, 279.89±2.15 and 440.46±1.79 days, respectively and dropped their first kids at
395.25±1.45, 469.73±2.68 and 618.68±1.84 days, respectively. The average size of litter in
female Keonjhar, Black Bengal and Ganjam goats were found as 1.77±0.47, 1.97±0.73 and
1.02±0.14, respectively. All the conformation and reproductive traits varied significantly
(p<0.05) among the three goat populations. However, Keonjhar and Black Bengal goats
didn’t show any significant difference with regard to tail length and gestation length.
Keonjhar goats showed significantly lower estimates for all the conformation traits than other
two goat populations with satisfactory reproductive performance. DNA isolation was carried
out from the buccal swab samples of Keonjhar goats and the complete coding region of the
Nerve Growth Factor (NGF) gene was amplified using the specified primers. Sequence
alignment with the reference sequence confirmed the presence of a synonymous mutation
(C291A) in this population. The gene frequencies of C and A allele were found to be 0.74 and
0.26. The does with CA genotypes showed higher litter size than CC genotype (p<0.05). The
findings indicate that NGF gene can be put to use as a candidate gene for the improvement of
the size of the litter in these population of goat. The nucleotide sequence of NGF gene in goat
is in close similarity with sheep, cattle, buffalo and wild yak. The value of theoretical
isoelectric point (PI), instability index, aliphatic index and GRAVY value for NGF protein
were predicted as 9.81, 45.73, 74.90 and -0.287, respectively. The secondary and tertiary
structure of NGF protein were predicted as well. Ten putative interacting proteins of goat
NGF protein were predicted.
Description
TH - 6646