Detection of organophosphate resistance in house flies population (Musca domestica L.) by laboratory bioassay method
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Date
2021
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Abstract
House fly menace is one of the major problems in poultry industry in the recent years. High density of
flies not only cause stress to birds and farmworkers, they can also cause great annoyance to nearby
human habitations which poses a serious public health problem. Adult house flies were collected from
five selected poultry farms using sweep net and reared in laboratory. Three to five days old F1 generation
flies produced by the healthy parental population, maintained in the laboratory were used for topical and
contact residual bioassays. In this study, different concentrations of dichlorvos were used for topical
bioassay such as, 0.02, 0.04, 0.08, 0.1, 0.2, 0.4 and 0.8 μg (a.i)/μl and 0.14, 0.28, 0.56, 1.12, 2.24 and
4.48 μg (a.i)/cm2 for residual contact bioassay. Mild to moderate resistance was observed in F1
generation house flies treated with dichlorvos from all the five farms. The resistance ratio ranged
between 1.46 to 15.15 and 6.23 to 22.45 fold by topical and residual contact bioassay respectively. In the
present study to identify the level of insecticide resistance in house flies to common insecticides by
laboratory bioassay methods.
Description
TNV_PIJ_2021_SP 10(5)830-835
Keywords
Veterinary Science