Estimation and Quantification of antimicrobial residue in poultry meat available in and around Jammu region
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Date
2022-01-14
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The antimicrobials like sulphaquinoxaline (SQX) and amprolium (AMP) are used indiscriminately in the poultry sector in Jammu region, J&K (UT). Therefore, the present study was conducted to quantitate SQX and AMP residue status in chicken tissue. Altogether, 20 samples each, collected from Jammu, Kathua, Reasi, Samba, Udhampur for residue analysis of SQX and 15 samples each from all except Udhampur for AMP residues estimation. Each sample included liver, kidney, and muscles. The RP HPLC UV method was used to estimate SQX and AMP residues at 266 and 263nm, respectively. The mobile phase was 0.01M ammonium acetate (pH 4.6), acetonitrile (43:57), flow at 1ml/minutes, peak Rt at 3.813 min. Recoveries of SQX from the liver, muscle, and kidney of chicken, spiked at 0.3 µg/g were 77.484 0.313, 86.838 0.050 and 78.900 0.134%, respectively. LOD and LOQ for SQX were 15.427 ng/ml and 46.749 ng/ml, respectively. The value for r2 was 0.999, showed good linearity. AMP was analyzed using phosphate buffer and acetonitrile (30:70; v/v) as mobile phase with peak Rt at 2.663min. Recoveries of AMP from the liver, muscle, and kidney of chicken, spiked at 400 ng/g were 72.1560.704, 71.0290.575, 68.3161.495%, respectively. LOD and LOQ for amprolium were 145 ng/ml and 441 ng/ml, respectively. The r2=0.999 exhibited good linearity. Out of the samples collected, 70% of the liver, 10% of kidney, and 25% of muscles from district Jammu had residue levels beyond established MRL. All the liver samples from Udhampur had deposition of SQX beyond the established MRL. Most tissue samples were positive; however, 50%, 25%, and 45% of the liver samples from Samba, Reasi, and Kathua, respectively, were found SQX residue beyond the prescribed MRL. Most of the tissue samples collected from various districts of Jammu were positive; however, none of the tissues exhibited values beyond established international MRL for AMP residue.