Studies on role of some non-enzymatic anti-oxidant additives on the cryopreservation of bovine semen
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Date
2021-12-20
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The present study was undertaken at Division of V.G.O., F.V.Sc & A.H, SKUAST-J, R.S. Pura, Jammu in collaboration with CABS, Hakkal, Jammu The main objective of this study was to ascertain the effects of different antioxidants (Ascorbic acid, Vitamin-E, Cysteine, Taurine, BHT and Trehalose) on cryopreservation of semen. Semen samples from cattle bull were used to evaluate the effect of additives on cryopreservation of semen. The complete experiment was divided into 3 groups. Group I: Control, TEYCAFG+ cysteine, TEYCAFG+ taurine. Group II: Control, TEYCAFG+ ascorbic acid, TEYCAFG+ vitamin E. Group III: Control, TEYCAFG+ trehalose, TEYCAFG+ BHT.
The results indicated that the progressive motility with taurine in group I, ascorbic acid and vitamin E in group II differed significantly (P<0.05) from control. The live sperm count with taurine in group I, ascorbic acid and vitamin E in group II differed significantly (P<0.05) from control. The acrosomal integrity at post-thaw stage with cysteine and taurine in group I, ascorbic acid in group II differed significantly (P<0.05) from control. The HOST with ascorbic acid in group II differed significantly (P<0.05) from control. Lipid peroxidation with cysteine and taurine in group I, ascorbic acid and vitamin E in group II and BHT in group III differ significantly (P<0.05) from control. The catalase with taurine in group I differ significantly (P<0.05) from control and cysteine, ascorbic acid in group II and both trehalose and BHT in group III differ significantly (P<0.05) from control. SOD with taurine in group I, ascorbic acid in group II & both trehalose and BHT in group III added semen differed significantly (P<0.05) from control. It was concluded that in group I the taurine, in group II ascorbic acid and in group III BHT added semen sample improved the quality of post-thaw semen though the increase was non-significant.