Induced Mutagenesis in the Introgression Lines to Improve Resistance to Foliar Diseases and Productivity in Groundnut (Arachis hypogaea L.)
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Date
2017-11
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University of Agricultural Science, Dharwad
Abstract
Present investigation was carried out to develop and evaluate induced mutants for superior productivity traits and resistance to foliar diseases in groundnut. M0 seeds of IL 3 and IL 4 were mutagenized with gamma rays (200 Gy and 300 Gy) and sodium azide (2 mM and 3 mM). The M1 seeds were sown during post-rainy season 2015 at IABT garden (E115), MARS, UAS, Dharwad. In M1 population, increasing dose of gamma rays led to reduction in germination and increase in lethality. But the increasing dose of sodium azide resulted in increased germination.
The M2 generation was raised during the rainy season of 2016. Both IL 3 and IL 4 recorded the score of 5 for ELS and 3 for LLS. ELS score ranged from 3 to 6 for both IL 3 and IL 4 derived mutants. LLS score ranged from 2 to 4 in IL 3 derived mutants and 1 to 4 in IL 4 derived mutants. In IL 3 and IL 4, the mutation frequency increased with increase in concentration of the mutagens and mutagenic effectiveness decreased with increase in dose of the mutagens individually and in combination. IL 3, IL 4 and their selected mutants were subjected to ddRAD-Seq to discover SNPs and copy number variations (CNVs). Number of SNPs varied from 2 to 12 in IL 3 derived mutants and 1 to 9 in IL 4 derived mutants.
Total 86 M3 lines were evaluated during post-rainy season 2016. Genotypes showed significant difference for majority of traits except for sound mature kernel weight percentage. All the M3 lines and parents showed resistant type of allele at all the foliar disease resistance-linked marker loci. Four IL 3 derived M3 lines were found superior for productivity over parent and can be tested under multi-locations for variety development.