Biological Hardening of Micropropagated Plantlets in Potato (SolanumtuberosumL.)
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Date
2021-12
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The present investigation entitled “Biological Hardening of Micropropagated Plantlets of Potato (SolanumtuberosumL.)” was conducted at Plant Tissue Culture Lab, Division of Vegetable Science & Floriculture, SKUAST-Jammu during the year 2020-21with the objectives (i) To refine protocol for rapid in vitroshoot multiplication in potato and (ii) To study the effect of bio-agents on hardening of micro-propagated potato plantlets. Two explants viz., sprouts and nodal segments of potato were used for the culture establishment.Standardisation of sterilization procedure of explants was carried out using different treatment durations of Bavistin (0.2%) + Streptomycin (200ppm) (5, 10 and 20 minute) followed by treatment with NaOCl (2%) (1, 2, 3 and 5 minute) and 10 minute pre-treatment with Bavistin (0.2%) + streptomycin (0.2%) followed by 2 minute treatment with NaOCl (2%) gave highest survival percentage for both the explants viz., nodal segments (87.78%) and sprouts (81.11%). Refinement of micropropagation protocol was carried out using MS medium fortified with different hormonal concentrations and combinations of BAP (1, 1.5 and 2 mg/l), TDZ (0.25, 0.50, 0.75 and 1mg/l) and IBA (0.5, 1, 1.5 and 2 mg/l).MS basal medium in combination with 1mg/l BAP and 0.25 mg/l TDZ was found superior in terms of early shoot induction and percentage response in both explants viz., nodal segment explants (8.11days and 88.89%, respectively) and sprouts (10.44 days and 76.67%, respectively). Least number of days for multiple shoot formation (21.22), maximum number of shoots per explant (7.22), maximum shoot length (7.28 cm) and maximum number of nodes per shoot (7.07) were also observed in MS basal medium in combination with 1mg/l BAP and 0.25mg/l TDZ. For induction of rooting healthy in vitro multiplied shoots were cultured on MS basal medium and half MS basal medium fortified with different concentrations of IBA ( 0.5, 1.0, 1.5 and 2.0 mg/l). Half MS basal medium in combination with 1.0 mg/l IBA recorded significantly superior responses in terms of percentage rooting (78.89%), number of roots per plantlet (9.34). Maximum root length (7.12 cm) was observed in Half MS basal medium in combination with 0.5 mg/l IBA.
Forbiological hardening studies Piriformosporaindica.Trichodermaviride, Pseudomonas fluorescens, Bacillus subtilis and consortia of Bacillus subtilis and Pseudomonas fluorescensalone and in combination with glycerol (0.5%) were used. Bio-agents were applied as root dip for 3 minutes and glycerol was applied by dipping the shoot portion of the plantlet in 0.5% glycerol solution. Trichodermaviridealong withglycerol (0.5%) application gave highest plantlet survival (73.33%) and all the treatments were found superior to the untreated control. Application ofPiriformosporaindicawithglycerol(0.5%) resulted in maximum increase in root length (56.93%) whereas,highest increase in plant height (72.78%) and internodal length (20.64%) was observed when consortia (Pseudomonas fluorescens + Bacillus subtilis) was used along with glycerol(0.5%).
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Preferred for your workNaik, R. 2021. Biological Hardening of Micropropagated Plantlets in Potato (Solanum tuberosum L.). M.Sc. thesis, Skuast-Jammu