Superovulatory response and embryo yield in buffaloes (Bubalus bubalis)
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Date
2020
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Abstract
Murrah graded pluriparous buffaloes (n=10) were subjected for three superovulatory treatments.
Treatment I (Control; n=20): Follicle Stimulating Hormone (600 mg) was initiated on the Day 10 for a
period of five days. Treatment II (SO-OS; n = 7): Superstimulatory treatment was initiated after
sychnronization of oestrous cycle by Ovsynch protocol. Gonadotrophin treatment for superovulation was
initiated on Day 6 of synchronized cycle. Treatment III (SO-FWS; n = 7): GnRH (Inj. Receptal; 10 μg
i.m.) was administered to all the animals on Day 6 of the cycle (Day 0: natural oestrus) and FSH
treatment was initiated 96 h after GnRH (Day 10). Animals were inseminated thrice with frozen thawed
proven during superovulatory oestrum and embryos/ova were recovered non-surgically on Day 5.5 of the
superovulatory cycle. Six animals (30.0%) in control group did not respond for the treatment, while all
the animals (100%) in SO-OS and SO-FWS groups responded for superovulation treatment. The mean
number of CL is significantly (P < 0.05) higher in SO-OS group than the other groups. The control and
SO-FWS groups had significantly (P < 0.01) greater number of anovulatory follicles (AFs). More
percentage of transferrable quality embryos were recovered from SO-OS group, while more number of
unfertilized ova and poor quality embryos were recovered from control and SO-FWS groups. It could be
concluded that superovulatory response, embryo yield and embryo quality are better in buffaloes
superovualted after the Ovsynch protocol.
Description
TNV_JEZS_2020_8(6)1468-1470
Keywords
Veterinary Science