“Characterization of Escherichia coli from sheep and goat for virulence factors and their antibiotic resistance with special reference to extended spectrum β lactamases from Jammu region.”
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Date
2021-10-20, 2021-10-20
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The study was aimed at investigating the pathogenic strains of Escherichia coli in sheep and goats and their antibiotic resistance with special reference to extended spectrum beta lactamases. Out of total 120 faecal samples, 200 presumptive E. coli isolates were collected. Out of 200 E. coli isolates 62 (31%) showed the presence of at least one virulence gene studied. Out of these 62 isolates, 10 (16.12%) isolates carried eaeA gene either alone or along with ehxA and were detected as EPEC, 44/62 (70.96%) isolates carried the stx gene and were detected as STEC. 08/62 (12.90%) isolates carried ehxA and were designated as EHEC. Out of 62 isolates which were screened for ESBL genes, 22 (35.48%) isolates were tested positive for presence of ESBL genes and the prevalence of blaTEM, blaCTX-M and blaSHV genes were 54.54%, 27.27% and 36.36% respectively. Antibiotic sensitivity profiling of the isolates positive for virulence genes revealed that all were resistant to cefotaxime, 95.16% to ceftazidime, 88.7% to cefoperazone, 88.2% to cefipime, and 69.3% to ciprofloxacin in the current investigation. All of the isolates were sensitive to chloramphenicol, 91.9% to doxycycline hydrochloride and 88.7% to amoxicillin/clavulanic acid.
The study was aimed at investigating the pathogenic strains of Escherichia coli in sheep and goats and their antibiotic resistance with special reference to extended spectrum beta lactamases. Out of total 120 faecal samples, 200 presumptive E. coli isolates were collected. Out of 200 E. coli isolates 62 (31%) showed the presence of at least one virulence gene studied. Out of these 62 isolates, 10 (16.12%) isolates carried eaeA gene either alone or along with ehxA and were detected as EPEC, 44/62 (70.96%) isolates carried the stx gene and were detected as STEC. 08/62 (12.90%) isolates carried ehxA and were designated as EHEC. Out of 62 isolates which were screened for ESBL genes, 22 (35.48%) isolates were tested positive for presence of ESBL genes and the prevalence of blaTEM, blaCTX-M and blaSHV genes were 54.54%, 27.27% and 36.36% respectively. Antibiotic sensitivity profiling of the isolates positive for virulence genes revealed that all were resistant to cefotaxime, 95.16% to ceftazidime, 88.7% to cefoperazone, 88.2% to cefipime, and 69.3% to ciprofloxacin in the current investigation. All of the isolates were sensitive to chloramphenicol, 91.9% to doxycycline hydrochloride and 88.7% to amoxicillin/clavulanic acid.
The study was aimed at investigating the pathogenic strains of Escherichia coli in sheep and goats and their antibiotic resistance with special reference to extended spectrum beta lactamases. Out of total 120 faecal samples, 200 presumptive E. coli isolates were collected. Out of 200 E. coli isolates 62 (31%) showed the presence of at least one virulence gene studied. Out of these 62 isolates, 10 (16.12%) isolates carried eaeA gene either alone or along with ehxA and were detected as EPEC, 44/62 (70.96%) isolates carried the stx gene and were detected as STEC. 08/62 (12.90%) isolates carried ehxA and were designated as EHEC. Out of 62 isolates which were screened for ESBL genes, 22 (35.48%) isolates were tested positive for presence of ESBL genes and the prevalence of blaTEM, blaCTX-M and blaSHV genes were 54.54%, 27.27% and 36.36% respectively. Antibiotic sensitivity profiling of the isolates positive for virulence genes revealed that all were resistant to cefotaxime, 95.16% to ceftazidime, 88.7% to cefoperazone, 88.2% to cefipime, and 69.3% to ciprofloxacin in the current investigation. All of the isolates were sensitive to chloramphenicol, 91.9% to doxycycline hydrochloride and 88.7% to amoxicillin/clavulanic acid.