XENOGENIC DECELLULAR DIAPHRAGMATIC SCAFFOLD FOR PERINEAL HERNIOPLASTY IN DOG
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Date
2018
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Nanaji Deshmukh Veterinary Science University Jabalpur
Abstract
Perineal hernia occurs commonly in middle aged or aging intact male dogs and rarely in females. Currently available synthetic biomaterials are not free from complications. On the other hand biological materials may result in significant reaction and rejection. With these fact present study was planned to standardize the procedure for placement of xenogenic decellular bubaline diaphragmatic scaffold for repair of perineal hernia in dogs and compare it with synthetic polygalactin and polypropylene composite mesh for repair of perineal hernia in dogs. During the study period 13568 cases were registered in TVCC from July 2017 to March 2018. Out of which 21 cases were diagnosed to be hernia. In these 21 cases only 12 cases showed the perineal hernia. Incidence of hernia in TVCC was 0.15% and Incidence of perineal hernia in TVCC was 0.09%.
In phase I decellular buffalo diaphragm was prepared using 1 % sodium dodecylsulphate (SDS) as a detergent and stored at -20o C till use. In phase II 12 dogs suffering with perineal hernia were randomly divided in to two equal groups. In of group I perineal hernioplasty was done with Synthetic polygalactin and polypropylene composite mesh while in group II, with decellular bubaline diaphragmatic scaffold (DBDS).
In group II three different techniques viz Onlay, Inlay and Sandwich methods were evaluated. In onlay and inlay method chance of recurrence and SSI (surgical site infection) was high as compare to sandwich method. Exudation and irritation was more in onlay method. Self mutilation was observed in onlay method. Mean surgical time was 59.17±14.04 and 57.50±6.15 minute in group I and II respectively. Rectal temperature within the normal range for all dogs. Swelling and redness was not seen on day 0 in both the groups which increased significantly (p0.05) between and within the groups.
Colour Doppler ultrasonographic examination revealed no vascularization on 0 day in both the groups. Neovascularuzation was observed on day 30 in group I and day 14 in group II on Colour Doppler ultrasonographic examination. It was increase on day 90 in both the groups.
The complication encountered in the present study includes local seroma, suture dehiscence self mutilation, constipation, fistula formation. Due to these finding, it can be conclude that sandwich technique was found to be more suitable in comparison to onlay an inlay technique. DBDS is well accepted by all animals and showed less complication.
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