Mapping of Yellow Vein Mosaic Virus Resistance in Okra
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
India is world’s largest producer of okra with 62 per cent share in world agriculture.
However, the productivity of okra is quite low. Yellow vein mosaic virus (YVMV) is a major
constraint in okra production. Infection of 100 per cent plants in a field is quite usual with
yield loss ranging between 50 and 94 per cent. In this study an attempt has been made to map
the gene(s) related to yellow vein mosaic virus resistance in okra in two different populations.
One population was developed through single seed descent method by crossing YVMV
susceptible A. esculentus var. Punjab Padmini and YVMV resistant okra wild species, A.
tuberculatus. 118 seeds of BC1F2 generation were sown and further selfed to produce 102
lines of BC1F2:3 generation. Screening for YVMV disease was done on 102 lines of BC1F2:3
generation. Phenotypic data revealed that YVMV resistance is controlled by single recessive
gene. 441 EST based SSR markers were amplified on parent plants to observe parental
polymorphism out of which 85 markers were identified as polymorphic. These markers were
further amplified on 118 plants of BC1F2 generation. 42 markers out of 85, showed distorted
segregation. Second population was developed by crossing A. esculentus var Punjab
Padmini with YVMV resistant okra wild species, A. manihot. Genotyping was done on
178 plants of BC1F2 generation. 239 EST based SSR markers were amplified on parent plants
to observe parental polymorphism. 70 markers were identified as polymorphic. These
markers were amplified for bulked segregant analysis between the two bulked DNA samples
along with the parental DNA samples. Four primer pairs i.e. Okra 271, Okra 300, Okra 324
and Okra 362 were found linked to YVMV disease. These markers were subsequently tested
for open bulked segregant analysis. Out of these, Okra 271 marker did not show specific
alignment with parental samples, and Okra 300, Okra 324 and Okra 362 showed alignment
with female parent. Thus, distorted segregation was observed in second population also.
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Citation
Mamta (2021). Mapping of Yellow Vein Mosaic Virus Resistance in Okra (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.